副猪嗜血杆菌qseC基因缺失突变对生物被膜形成的影响

doi:10.11843/j.issn.0366-6964.2017.08.014

Abstract

Abstract:

The qseC gene mutation of Haemophilus parasuis was constructed to investigate the effect of qseC gene on the growth and biofilm formation of H. parasuis. The qseC mutant of H. parasuis was constructed by natural transformation. The growth curves of wild-type strain SC096 and △qseC were depicted. Then crystal violet staining was used to test biofilm formation of wild-type strain and qseC mutant. The amount of biofilm of SC096 and △qseC were measured by a 96-well plate quantitative method. The biofilm changes of the two strains were then observed by scan electron microscope. Results were as follows:The qseC gene mutant of H. parasuis SC096 was constructed successfully. The growth curves revealed that the growth rate of SC096 was similar to that of its qseC mutant. The biofilm formation ability of △qseC was weaker than that of the wild type strain SC096. The results showed that qseC gene may play an important role in the regulation of H. parasuis biofilm formation.

CLC Number:

S852.613

YANG Kai-jie, FENG Sai-xiang, ZHOU Qi, ZHANG Jian-min, LIAO Ming, FAN Hui-ying. Effect of qseC Gene Mutation of Haemophilus parasuis on Biofilm Formation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1499-1504.

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Effect of qseC Gene Mutation of Haemophilus parasuis on Biofilm Formation

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